In this online course you will get an excellent training in analysis of RNA-sequencing from basic theoretical topics to practical analysis of raw data. First you will learn the primary concepts including the different types of coding and none-coding RNA molecules and the technical methods of RNA sequencing. Then you will practice to explore RNA-seq databases manipulate datasets and download raw RNA-seq reads.You can also practice how to check the overall quality of raw reads using the FastQC and Trimmomatic packages. We also teach alignment procedure using HISAT2 and read counts using HTSeq packages. Additionally you can gain deep insights into RNA-seq file formats (FASTQ SAM and BAM) and Samtools. Practicing with Linux and terminal environments is another benefit of this course.In addition to the scientific content we paid special attention to the teaching style for effective learning. For better learning we have tried to present all the steps in visual form during the course. You can brush up your RNA-seq analysis skills with our practical tutorials and hands-on exercises without getting involved with a large volume of theoretical and boring information. Enroll in this course now.